Comparison of culture and PCR for the detection of Brucella melitensis in blood and lymphoid tissues of serologically positive and negative slaughtered sheep

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Ilhan Z., Aksakal A., Ekin İ. H., GULHAN T., SOLMAZ H., ERDENLIG S.

LETTERS IN APPLIED MICROBIOLOGY, cilt.46, sa.3, ss.301-306, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 46 Sayı: 3
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1111/j.1472-765x.2007.02309.x
  • Dergi Adı: LETTERS IN APPLIED MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.301-306
  • Anahtar Kelimeler: blood, Brucella melitensis, culture, lymphoid tissue, PCR, sheep, SINGLE-STEP PCR, ABORTUS, MILK, DNA, DIAGNOSIS, CATTLE, GOATS
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Aims: To compare the culture and PCR methods for detection of Brucella melitensis in blood and lymphoid tissue samples obtained from slaughtered sheep (n = 162) testing positive/negative in serological tests (Rose Bengal test and serum agglutination test).

Aims: To compare the culture and PCR methods for detection of Brucella

melitensis in blood and lymphoid tissue samples obtained from slaughtered

sheep (n = 162) testing positive ⁄ negative in serological tests (Rose Bengal test

and serum agglutination test).

Methods and Results: Of 162 sheep examined, 45 were positive and 117 nega-

tive in serological tests. A PCR assay based on a pair of Br. melitensis-specific

primers was used to detect DNA in blood and lymphoid tissue. Brucella melit-

ensis was isolated from 1Æ2% (2 ⁄ 162) and 17Æ2% (28 ⁄ 162) of the blood and

lymphoid tissue samples respectively. Positive PCR products with a molecular

size of 731 bp were obtained from 27Æ7% (45 ⁄ 162) of blood and 29Æ0%

(47 ⁄ 162) of lymphoid tissue samples.

Conclusions: The species-specific PCR assay detected a higher number of Br.

melitensis DNA both from serologically positive (P <0Æ01 in blood PCR,

P <0Æ001 in tissue PCR) and serologically negative (P <0Æ001 in both blood

PCR and tissue PCR) sheep compared with classical bacteriological culture

methods.

Significance and Impact of the Study: The results emphasize the importance of

using more than one type of diagnostic technique for the detection of animals

positive for brucellosis, especially with epidemiological purposes.