In this study, we aimed to determine the antigenetoxic and antioxidant capacity of total extract of two lichens against to Aflatoxin B-1 (AFB(1)). For this aim, we obtained the total extracts from Letharia vulpina (LTE) and Vulpicida pinastri (VTE) lichens species. AFB(1) induced oxidative stress and genotoxicity in human lymphocytes in vitro. Our results showed that 5 mu M concentration of AFB(1) increased the frequency micronucleus (MN) and malondialdehyde (MDA) levels, decreased the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione (GSH) level. On the other hand, cotreatment of 5, 10 and 20 mu g/ml concentrations of lichen extracts with AFB(1) decreased the frequency of MN and MDA level and activities of SOD, GPx and GSH level increased. The results of the experiments have clearly shown that LTE and VTE have strong antioxidative and antigenotoxic effect in human lymphocytes in vitro.