Scientia Parasitologica, cilt.26, sa.3-4, ss.81-97, 2025 (Hakemli Dergi)
Tick-borne diseases, particularly anaplasmosis, have a significant impact on livestock production. This study aimed to investigate the incidence and prevalence of Anaplasma ovis and Anaplasma capra in sheep herds in the Gevaş district of Van Province, Türkiye. PCR analysis was performed on blood samples from 200 clinically healthy sheep using msp4 and gltA gene-targeted PCR primers. A total of 149 (74.5%) samples tested positive for A. ovis, whereas none were positive for A. capra. No significant associations were observed between A. ovisinfection and host factors such as sex and age (P > 0.05). However, its prevalence was higher in the İnköy region (P < 0.05). Sanger sequencing confirmed partial msp4 gene sequences, which were submitted to GenBank (accession numbers PV231424-PV231428). The A. ovis isolates showed low genetic diversity compared to the strains from other countries. This study highlights the high prevalence of A. ovis in the region, in contrast to the absence of A. capra. Therefore, further research is needed to focus on the epidemiology and impact of A. capra.