Keçi Sütüne Eklenen Farklı Süt Türlerinin Belirlenmesi İçin PCR Yöntemlerinin Karşılaştırılması

Tuncay R. M., Sancak Y. C.

Balikesir Health Sciences Journal , cilt.11, sa.3, ss.462-470, 2022 (Hakemli Dergi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.53424/balikesirsbd.1139179
  • Dergi Adı: Balikesir Health Sciences Journal
  • Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM), Index Copernicus, Sobiad Atıf Dizini
  • Sayfa Sayıları: ss.462-470
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Objective: This study, it was aimed to determine which of the multiplex conventional PCR and Real-Time PCR methods 

are more suitable for the detection of cow and sheep milk mixed with goat milk. Materials and Methods: For this purpose, 

one liter of each goat, cow, and sheep milk was obtained from farms in Van province. PCR experiments were carried out by 

adding cow's milk and sheep's milk in the same proportions into goat milk (1%, 2%, 5%, 0.1%, and 0.5%). Multiplex 

conventional and Real-Time PCR were used in these trials. Results: In the cow and sheep milk trials, it was determined that 

the presence of 1%, 2%, and 5% cow and sheep milk added to goat milk could be determined by the multiplex conventional 

PCR method. However, it was observed that the positivity of the gel image of the milk mixtures added at the rate of 0.5% 

was unclear, and the mixtures at the rate of 0.1% could not be detected. In the Real-Time PCR method, the presence of cow 

and sheep milk was detected in all the mixtures and positive graphics were determined. Conclusion: This showed that the 

Real-Time PCR method gives more reliable results even when 0.1% cow or sheep milk is mixed with commercially available 

goat milk.