Van Yuzuncu Yil University 1st International Health Sciences Congress (Vusak 2022), Van, Turkey, 27 - 29 October 2022, vol.1, no.4, pp.24
Objective: In this study, it was aimed to measure the level of expression of important marker genes (RIPK 1 and RIPK 2) on the necrotic
pathway resulting from the damage caused by fluorosis in the rat liver, and to investigate the potential effects of antioxidant-featured
Vitamin C and Vitamin E on reducing the damage that may ensue.
Material-Method: In the study, 48 male rats of the Wistar-Albino strain weighing 200–250 g were used. A total of 6 groups, namely the
control group, corn oil, NaF, NaF+vitamin E, NaF+vitamin C, and the NaF+vitamin C+vitamin E groups, each comprising 8 rats, were
formed. NaF was added to the drinking water of the animals as 150 mg/kg for a duration of 16 weeks. In the treatment groups, after
having administered Naf for 16 weeks, vitamin C (100 mg/kg), vitamin E (300 mg/kg) and vitamin C+vitamin E (100 mg/kg+300 mg/kg)
were administered for 4 weeks.
Results: At the end of the experiment, the necrotic gene expressions were determined in the Liver RNA isolation products in all groups.
The differences between the groups were compared with the fold-changes coefficient in the increase/decrease in the control gene (ACTB)
expression and evaluated.
Conclusion: As a result, it was determined that fluorosis had triggered the necrotic gene expression and increased the degree of
expression. It was determined that the vitamins administered for treatment had reduced the increased gene expression levels one on one,
but that when used in a combined manner, it had provided a reduction close to the control.