This study was carried out to investigate the effects of different sulphur doses (0, 25 and 50 kg ha 1) on the yield and agronomic characteristics of three spring rapeseed cultivars (Gladiator, Licosmos and Jura) in eastern Anatolia, during 2010 and 2011. Field trials were designed according to randomized complete block design (RCBD) with three replications at the experimental fields of Faculty of Agriculture, Van Yuzuncu Yil University, Van, Turkey. In the study, plant height (cm), number of branches (branch plant(-1)), number of pod (pod plant-1), number of seed in the pod (seed pod(-1)), thousand-seed weight (g), seed yield (kg ha(-1)), oil content (%), oil yield (kg ha(-1)) and protein content (%) were determined. The effect of sulphur doses on the studied characters was statistically significant. However, statistically significant differences were not observed among different cultivars except plant height, number of branches, number of seed in the pod, seed yield, oil yield and protein content. The highest seed yield (1418.0 kg ha(-1)) and oil content (41.1 %) were obtained using 25 kg ha(-1) sulphur application for cv. Gladiator in second year field experiment.