In this study, the goal was the germination and propagation of Orchis sancta L. which is endangered due to uncontrolled collection and used in obtaining salep, in vitro conditions. By the addition of 0 (control group), 20, 40, 60, 80, and 100 g/l concentrations of sucrose, glucose, maltose, galactose, and fructose of carbohydrate forms into Van WaesDebergh culture medium, sugars of effects were determined on germination and development of Orchis sancta L. seeds. In the study, germination, formation of protocorm and shoot periods and ratios were examined. The highest average germination ratio was obtained as 77.85% in Maltose40, the lowest average as 44.36% in Galaktose100 mediums. In protocorm formation, when the highest average ratio was detected as 68.53% in Sucrose100, the lowest average as 25.33% in Fruktose100 mediums. Germination period as 12.50-21.33 days, protocorm formation period as 22.50-50.83 days and shoot formation period as 50.66-105.33 days' intervals were determined.