This study aimed to examine the surface characteristics of low shrinkage composites and adhesion of Streptococcus mutans and Streptococcus mitis to these materials. Control material (glass) and three low shrinkage composites (Charisma Diamond, Kalore GC, Beatiful II LS) were used. After polishing procedure was applied to composite specimens, surface roughness (SR), surface free energy (SFE), and contact angle measurements were performed. Surfaces of composite were analyzed using scanning electron microscope and energy-dispersive X-ray spectroscopy. After pellicle formation with artificial saliva, S. mutans and S. mitis biofilms were incubated in 5% CO2 for 24 h at 37 degrees C and were analyzed using confocal laser scanning microscopy. The lowest SR and highest SFE values were found in the control group. While the contact angle of control was statistically lower than composites, statistically difference was not found between composite groups. S. mutans adhesion of composites was significantly lower than control group, but there was no significant difference between composites. S. mitis adhesion of all groups was statistically similar. SR did not affect the S. mutans and S. mitis adhesion. Less adherence of S. mutans to low shrinkage composites was associated with low SFE and high contact angle values. Even though the highest SR was observed in the Charisma Diamond, no difference was found between the composites in terms of bacterial adhesion.