Research Information System
Ecological Life Sciences, vol.17, no.4, pp.170-178, 2022 (Scopus)
Among the parasitic disease agents, Diplostomums are among the most common and highly pathogenic agents. In this study, DNA isolation and subsequent purity and concentration differences were investigated from parasite samples taken under different stock conditions. In the concentration differences, it was observed that the concentrations of the parasite samples kept at -20°C without buffer and the samples kept in 0.9% saline at -20°C were insignificantly higher (p<0.05). According to the results of the study, if the starting material is DNA, it has been observed that there is no problem in terms of DNA concentration if the tissue samples are kept at -20°C in a short time (3 days) without the need for a buffer solution.