Detection of CryptosporidiumSpp. in Calves Through Nested PCR and Kinyoun’s Acid-Fast Methods in Van, Turkey
International Journal of Ecosystems and Ecology Science (IJEES), cilt.10, sa.2, ss.271-276, 2020 (ESCI)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 10 Sayı: 2
- Basım Tarihi: 2020
- Doi Numarası: 10.31407/ijees
- Dergi Adı: International Journal of Ecosystems and Ecology Science (IJEES)
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), CAB Abstracts, Environment Index, Veterinary Science Database
- Sayfa Sayıları: ss.271-276
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Van Yüzüncü Yıl Üniversitesi Adresli: Evet
Özet
Tetratrichomonas gallinarum is a protozoan parasite of various galliform birds causing a type of enterohepatitis
termed tetratrichomoniasis. During the year 2018-2019 an outbreak of unspecified granulomatous diseases,
characterized by the sudden deaths of young turkeys were observed in 18 turkey farms in the Van province of
Turkey. Three hundred eighty fecal samples were obtained from the infected farms and 42 tissue samples (liver and
caecum) collected from the dead turkeys were examined under light microscope and through molecular
characterization. The necropsies of dead turkeys revealed granulomatous necrotic foci in the caecum and liver. A
number of 125 (32.89%) fecal samples and all of the 42 (100%) tissue samples were found positive for T.
gallinarum by molecular characterization. The sequence analysis for both fecal and tissue samples were confirmed
with T. gallinarum (MH935510) those showed 97.9% similarity on the ‘Basic Local Alignment Search Tool’. This
is the first molecular characterization report of T. gallinarum in Turkey