Secondary Metabolites from Astragalus lycius and Their Cytotoxic Activities


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Horo I., Kocabas F., Alankus-Caliskan O., Özgökçe F., Khan I. A. , BEDIR E.

NATURAL PRODUCT COMMUNICATIONS, vol.11, no.12, pp.1847-1850, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 11 Issue: 12
  • Publication Date: 2016
  • Journal Name: NATURAL PRODUCT COMMUNICATIONS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1847-1850
  • Keywords: Astragalus lycius, Isoflavone, Lignan, Cytotoxic activity, Cancer, Chemotaxanomy, CYCLOARTANE TRITERPENE GLYCOSIDES, AERIAL PARTS, CHEMICAL-CONSTITUENTS, ROOTS, MICROCEPHALUS, SAPONINS, BOISS
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Eight known secondary metabolites were isolated from the methanolic extract of the whole plant of Astragalus lycius Boiss. They were identified as 5,5’dihydroxy-3’-methoxy-isoflavone-7-O? -D-glucoside (1), genistin (2), sissotrin (3), 5,4’-dimethoxy-isoflavone-7-O? -D-glucopyranoside (4), (7S,8R)-5methoxydehydrodiconiferyl alcohol-4-O? -D-glucopyranoside (5), 4-O-lariciresinol-glucoside (6), 2-phenylethyl? -D-glucopyranoside (7) and ? -sitosterol-3O-β-D-glucopyranoside (8) by spectroscopic methods including 1H- and 13C-NMR and HR-MS experiments, and by comparison with literature values. Compounds 1-7 are reported for the first time from Astragalus taxa. All of the compounds were tested for their cytotoxic activities against a number of cancer cell lines. Among them, only 6 exhibited significant activity against human colon carcinoma (HT-29) at 2.69 ?M concentration. 
 Keywords:  Astragalus lycius, Isoflavone, Lignan, Cytotoxic activity, Cancer, Chemotaxanomy. 

Eight known secondary metabolites were isolated from the methanolic extract of the whole plant of Astragalus lycius Boiss. They were identified as 5,5'-dihydroxy-3'-methoxy-isoflavone-7-O-beta-D-glucoside (1), genistin (2), sissotrin (3), 5,4'-dimethoxy-isoflavone-7-O-beta-D-glucopyranoside (4), (7S,8R)-5-methoxydehydrodiconiferyl alcohol-4-O-beta-D-glucopyranoside (5), 4-O-lariciresinol-glucoside (6), 2-phenylethyl-beta-D-glucopyranoside (7) and beta-isitosterol-3-O-beta-D-glucopyranoside (8) by spectroscopic methods including H-1- and C-13-NMR and HR-MS experiments, and by comparison with literature values. Compounds 1-7 are reported for the first time from Astragalus taxa. All of the compounds were tested for their cytotoxic activities against a number of cancer cell lines. Among them, only 6 exhibited significant activity against human colon carcinoma (HT-29) at 2.69 mu M concentration.