Evolution of slime production by coagulase-negative staphylococci and enterotoxigenic characteristics of Staphylococcus aureus strains isolated from various human clinical specimens


BOYNUKARA B., GULHAN T., Gurturk K. , ALISARLI M., Ogun E.

JOURNAL OF MEDICAL MICROBIOLOGY, cilt.56, ss.1296-1300, 2007 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 56 Konu: 10
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1099/jmm.0.47140-0
  • Dergi Adı: JOURNAL OF MEDICAL MICROBIOLOGY
  • Sayfa Sayıları: ss.1296-1300

Özet

The present study was designed to determine the slime production of coag u lase- negative staphylococci (CoNS) and the enterotoxigenic properties of Staphylococcus aureus strains, and to evaluate the clinical importance of slime-producing CoNS and enterotoxigenic S. aureus strains isolated from various human clinical specimens. For this purpose, a total of 120 Staphylococcus strains were isolated and identified, and further characterized for their slime production and enterotoxigenicity. Of the clinical isolates, 55 (45.8 %) were found to be S. aureus, and the others (54.2 %) were identified as CoNS. Of the CoNS, 20 (116.7 %) were further identified as Staphylococcus hominis, 18 (15 %) as Staphylococcus epidermidis, six (5 %) as Staphylococcus xylosus, six (5 %) as Staphylococcus warneri, five (4.2 as Staphylococcus sciuri, four (3.3 %) as Staphylococcus haemolyticus, and two each (1. 7 as Staphylococcus simulans, Staphylococcus capitis and Staphylococcus saprophyticus, respectively. Thirty-nine (60%) of 65 CoNS were found to be slime producers. Slime production was observed in all CoNS, except S. capitis, mostly from blood (38.5 %), tracheal aspiration (20.5 %) and urine (112.8 %) specimens. In addition, of the 55 S. aureus isolates, 46 (83.6 %) were found to be enterotoxigenic, and of these S. aureus strains, 39 (84.7 %) were positive for staphylococcal enterotoxin (SE)A. The results of this study showed that the slime-producing CoNS were mostly found in clinical specimens of blood, tracheal aspirate and urine. SEA was the predominant enterotoxin type detected in S. aureus strains from human clinical specimens.