CpG motif-based adjuvant as a replacement for Freund's complete adjuvant in a recombinant LHRH vaccine

Conforti V. A., De Avila D. M., Curnmings N. S., Zanella R., Wells K. J., Uelker H., ...Daha Fazla

VACCINE, cilt.26, sa.7, ss.907-913, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 7
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1016/j.vaccine.2007.12.010
  • Dergi Adı: VACCINE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.907-913
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

This study compared: (1) Freund's complete adjuvant and CpG oligodeoxynucleotide (ODN) 2006 in water-in-oil emulsion as adjuvants; and (2) increasing doses of a recombinant ovalbumin-LHRH (ova-LHRH) fusion protein as an antigen for a contraceptive vaccine. Treatment groups (n = 8 heifers/group) were: one untreated control group; five groups receiving CpG ODN with different doses of ova-LHRH (1.5; 2.3; 3.4; 5.1; and 7.6 mg); and one group receiving 3.4 mg ova-LHRH in Freund's. Heifers were immunized at weeks 0 and 14. Ail vaccine treatments caused gonadal regression and estrus suppression. CpG ODN is a suitable replacement for Freund's for LHRH immunization. (c) 2007 Elsevier Ltd. All rights reserved.