CpG motif-based adjuvant as a replacement for Freund's complete adjuvant in a recombinant LHRH vaccine


Conforti V. A. , De Avila D. M. , Curnmings N. S. , Zanella R., Wells K. J. , Uelker H. , ...More

VACCINE, vol.26, no.7, pp.907-913, 2008 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 7
  • Publication Date: 2008
  • Doi Number: 10.1016/j.vaccine.2007.12.010
  • Title of Journal : VACCINE
  • Page Numbers: pp.907-913

Abstract

This study compared: (1) Freund's complete adjuvant and CpG oligodeoxynucleotide (ODN) 2006 in water-in-oil emulsion as adjuvants; and (2) increasing doses of a recombinant ovalbumin-LHRH (ova-LHRH) fusion protein as an antigen for a contraceptive vaccine. Treatment groups (n = 8 heifers/group) were: one untreated control group; five groups receiving CpG ODN with different doses of ova-LHRH (1.5; 2.3; 3.4; 5.1; and 7.6 mg); and one group receiving 3.4 mg ova-LHRH in Freund's. Heifers were immunized at weeks 0 and 14. Ail vaccine treatments caused gonadal regression and estrus suppression. CpG ODN is a suitable replacement for Freund's for LHRH immunization. (c) 2007 Elsevier Ltd. All rights reserved.