A stereological study on determination of ventricular wall volume of the heart in female and male quails


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Çakmak g., karadağ h., soygüder z., RAĞBETLİ M. Ç., kara M., AKYOL V.

Mehmet Akif Ersoy Üniversitesi Veteriner Fakültesi Dergisi, cilt.8, sa.1, ss.8-14, 2023 (Hakemli Dergi) identifier

Özet

In this study, ventricular wall volume of female and male quails was investigated stereologically. Six females and six males quails were used in this study. All of the animals were perfused. After the per- fusion, the quails were kept in 10% formaldehyde solution. Afterwards, chests of quails were cut and their hearts were resected. Ventricles of the hearts were separated. Specific ratio of tissue samples was obtained from each ventricle. The 5-μm thick samples were cut by using a microtome. Sequenti- ally, 10 sections were obtained. These sections were stained by hematoxylin eosin and photographed. Volumes of wall of the ventricles were estimated by using the Cavalieri’s Principle. In this study, the volume values of ventricular walls of female and male quails were compared. Some differences were found between these values. The volume values of ventricular walls of six female quails were com- pared with each other. While the lowest volume value was 0.398 cm³, the highest volume value was 0.612 cm³. The volume values of the male quails were between 0.438 cm³-0.817 cm³. It was found that volume values of ventricular walls of male quails were higher than volume values of ventricular walls of female quails. As a result, although there was a specific distinction between volume values of ventricular walls of female and male quails. There was no difference between statistic values (P>0.05). It was thought that this study will be guiding for other related studies.