The effect of extracellular matrix on the differentiation of mouse embryonic stem cells

ÖZDİL BAY B., Guler G., Acikgoz E., Kocaturk D. C., AKTUĞ H.

JOURNAL OF CELLULAR BIOCHEMISTRY, cilt.121, sa.1, ss.269-283, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 121 Sayı: 1
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/jcb.29159
  • Dergi Adı: JOURNAL OF CELLULAR BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.269-283
  • Anahtar Kelimeler: cell fate, differentiation, FTIR spectroscopy, Matrigel, mouse embryonic stem cell, FTIR, OSTEOPONTIN, SPECTRA, RAMAN, SPECTROSCOPY, INHIBITION, COMMITMENT, DYNAMICS, ADHESION, PHASE
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Embryonic stem cells (ESCs) are promising research materials to investigate cell fate determination since they have the capability to differentiate. Stem cell differentiation has been extensively studied with various microenvironment mimicking structures to modify cellular dynamics associated with the cell-extracellular matrix (ECM) interactions and cell-cell communications. In the current study, our aim was to determine the effect of microenvironmental proteins with different concentrations on the capacity and differentiation capability of mouse ESCs (mESCs), combining the biochemical assays, imaging techniques, Fourier transform infrared (FTIR) spectroscopy, and unsupervised multivariate analysis. Based on our data, coating the surface of mESCs with Matrigel, used as an acellular matrix substrate, resulted in morphological and biochemical changes. mESCs exhibited alterations in their phenotype after growing on the Matrigel-coated surfaces, including their differentiation capacity, cell cycle phase pattern, membrane fluidity, and metabolic activities. In conclusion, mESCs can be stimulated physiologically, chemically, or mechanically to convert them a new phenotype. Thus, identification of ESCs' behavior in the acellular microenvironment could be vital to elucidate the mechanism of diseases. It might also be promising to control the cell fate in the field of tissue engineering.