Sequential modelling for carbohydrate and bioethanol production from Chlorella saccharophila CCALA 258: a complementary experimental and theoretical approach for microalgal bioethanol production

Onay M.

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, vol.29, no.10, pp.14316-14332, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 10
  • Publication Date: 2022
  • Doi Number: 10.1007/s11356-021-16831-w
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, IBZ Online, ABI/INFORM, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.14316-14332
  • Keywords: Interactive response surface modeling, Artificial neural network, Flocculants, Wastewater, Hydrogen peroxide, alpha-Amylase, amyloglycosidase, Fermentation, ARTIFICIAL NEURAL-NETWORK, RESPONSE-SURFACE METHODOLOGY, BIOMASS PRODUCTION, WASTE-WATER, OPTIMIZATION, FERMENTATION, PRETREATMENT, FLOCCULATION, FEEDSTOCK, BIODIESEL
  • Van Yüzüncü Yıl University Affiliated: Yes


Bioethanol production from microalgal biomass is an attractive concept, and theoretical methods by which bioenergy can be produced indicate saving in both time and efficiency. The aim of the present study was to investigate the efficiencies of carbohydrate and bioethanol production by Chlorella saccharophila CCALA 258 using experimental, semiempirical, and theoretical methods, such as response surface methods (RSMs) and an artificial neural network (ANN) through sequential modeling. In addition, the interactive response surface modeling for determining the optimum conditions for the variables was assessed. The results indicated that the maximum bioethanol concentration was 11.20 g/L using the RSM model and 11.17 g/L using the ANN model under optimum conditions of 6% (v/v %) substrate and 4% (v/v %) inoculum at 96-h fermentation, pH 6, and 40 degrees C. In addition, the value of the experimental data for carbohydrate concentration was 0.2510 g/g biomass at ANN with the maximums of 50% (v/v) wastewater concentration, 4% (m/m) hydrogen peroxide concentration, and 6000 U/mL enzyme activity. Finally, although the RSM model was more effective than the ANN model for predicting bioethanol concentration, the ANN model yielded more precise values than the RSM model for carbohydrate concentration.