Effect of thymoquinone administration on tissue total oxidant/antioxidant status in diabetic rats

Usta A., Dede S., Çetin S.

8. Ulusal Veteriner Biyokimya ve Klinik Biyokimya Kongresi (8.Vetbiyokimya-2016), Bursa, Turkey, 22 - 24 September 2016, vol.1, no.1, pp.86-87

  • Publication Type: Conference Paper / Full Text
  • Volume: 1
  • City: Bursa
  • Country: Turkey
  • Page Numbers: pp.86-87
  • Van Yüzüncü Yıl University Affiliated: Yes


Objective: In this study, the effects of thymoquinone (TQ) on total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were investigated in streptozotocin (STZ)-induced diabetic rats in the liver and kidney tissues

Material and Methods: For this purpose, 24 male Wistar-Albino rats, weighing between 200-250 g were used. The rats were divided into four groups of control (C), thymoquinone (T), diabetes (D) and diabetes+thymoquinone (DT) with six rats in each group. To create diabetes in the rats belonging to D and DT groups, 45 mg / kg single dose of streptozotocin (STZ) were administered intraperitoneally (ip). TQ was administered as 30 mg/kg/21 day by oral gavage to the rats in DT and T groups. The values of total antioxidant status (TAS) and total oxidant status (TOS) were determined in liver and kidney tissue samples in the end of the study.

Results: Kidney TAS and TOS levels were found to be highest in the TQ group, lowest in the diabetic group (p≤0.05). There were no differences in other groups. Liver TAS was found to be lowest in the diabetes group (p≤0.05). In the Diabetes+TQ group there was no difference from control and TQ groups. No difference was detected in liver TOS. OSİ, on the other hand, were determined significantly high in the tissues in both diabetic groups (p≤0.05) while it approached to the control group in the Diabetes+TQ group.

Conclusions: As a result, it was revealed that TAS / TOS of liver and kidney tissues in experimental diabetic rats were affected by the implementation of TQ. OSI, in the diabetes+TQ group, it was found that it approached to the control group.

Keywords: Experimental diabetes, oxidative stress index, thymoquinone, total antioxidant capacity, total oxidant capacity