EFFECT OF Rhabdosciadium anatolyi AND CHLOROGENIC ACID ON SERUM RETINOL, CHOLECALCIFEROL AND PHYLLOQUINONE LEVELS IN EXPERIMENTAL CYCLOPHOSPHAMIDE TREATED RATS

Bakir A., Ekin S., Firat M.

JOURNAL OF ANIMAL AND PLANT SCIENCES, cilt.35, sa.3, ss.1-15, 2025 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35 Sayı: 3
  • Basım Tarihi: 2025
  • Doi Numarası: 10.36899/japs.2025.3.0063
  • Dergi Adı: JOURNAL OF ANIMAL AND PLANT SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Business Source Elite, Business Source Premier, CAB Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.1-15
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

In this study, the effects of chlorogenic acid (CA), and ethanol extract of Rhabdosciadium anatolyi (R. anatolyi) flowers

on the levels of vitamins (retinol, cholecalciferol and phylloquinone) in the serum of experimentally cyclophosphamide

(CP)-induced rats were investigated. Wistar-albino female rats (N=48) of 8 weeks old (200-250 g) were divided into six

groups of 8 animals each (control (0.9% NaCl), 200 mg/kg CP, 100 mg/kg CA, 300 mg/kg R.anatolyi flowers extract,

200 mg/kg CP + 300 mg/kg R.anatolyi flowers extract and 200 mg/kg CP + 100 mg/kg CA). At the end of the 7-day

study, blood samples were collected from the rats’ heart and serum was extracted. Retinol, cholecalciferol and

phylloquinone levels in the groups were determined simultaneously using simple reversed phase HPLC method.

According to the results of statistical analysis, a significant difference (p<0.01, p<0.05) was observed between the

control group and the groups treated with 200 mg/kg CP and 200 mg/kg CP + 300 mg/kg R. anatolyi flower ethanol

extract, respectively. In addition, a significant difference (p<0.01) in cholecalciferol levels between control and 200

mg/kg CP groups was detected. However, no statistically significant difference (p>0.05) was observed between the

groups in phylloquinone levels. It is concluded that CP causes free radical damage by causing oxidative stress, and

retinol, is more effective and more resistant in the antioxidant defense system compared to cholecalciferol and

phylloquinone.