Aim. The purpose of this study was to investigate the effects of an eight-week resistance exercise program on blood fats and blood sugar in sedentary women. The study included 20 volunteers with a mean age of 31.70 +/- 3.81 years. Materials and methods. Blood samples were taken by the experts in the appropriate laboratory environment in the morning before and after the resistance exercise program, which was administered 60 minutes 3 days a week for 8 weeks in the mornings on an empty stomach. Dependent groups t-test (Paired Sample T-Test) was applied to compare descriptive statistics with pre- and post-exercise values by using SPSS 22.0 package program. Results. There was a significant difference between GLU, Triglyceride, HDL, LDL, and total cholesterol levels before and after the resistance exercise program (p > 0.05). Conclusions. Resistance exercises have a positive effect on blood sugar and blood fat change. Therefore, we think that it can be used as protection from cardiovascular diseases, and if these exercises are applied regularly, they will have many positive results in terms of health.