Effects of sunitinib on immunoreactivity of vimentin, E-cadherin and S100 in kidneys of streptozotocin induced diabetic mice

Akarca-Dizakar S. O., AKTUĞ H., OLTULU F., ÖKTEM G., YAVAŞOĞLU A., Acikgoz E., ...More

BIOTECHNIC & HISTOCHEMISTRY, vol.93, no.5, pp.328-335, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 93 Issue: 5
  • Publication Date: 2018
  • Doi Number: 10.1080/10520295.2018.1439532
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.328-335
  • Keywords: Diabetes mellitus, e-cadherin, kidney, mice, S100, streptozotocin, sunitinib, tyrosine kinase inhibitor, vimentin, CELL-ADHESION, EXPRESSION, GLUCOSE, BETA, NEPHROPATHY, MECHANISMS, PROTEINS, MELLITUS, MODELS, SYSTEM
  • Van Yüzüncü Yıl University Affiliated: No


Diabetes mellitus (DM) affects many organs including kidney. Tyrosine kinase can cause hypoglycemia and sunitinib is an inhibitor of tyrosine kinase. We investigated the possible effects of sunitinib on the kidney of streptozotocin (STZ) induced type 1 diabetic mice. We used 28 CD 1 type male mice divided into four groups of seven. Type 1 diabetes was induced by injection of STZ. Group 1 was the untreated control. Group 2 comprised non-diabetic mice + sunitinib. Both groups 1 and 2 exhibited normal blood glucose levels. Group 3 comprised STZ treated diabetic mice + saline. Group 4 were diabetic mice + sunitinib treatment. Kidneys were removed after 8weeks. The immunoreactivities of vimentin, E-cadherin and S100 were assessed. Immunostaining of vimentin, E-cadherin and S100 was located in both the glomeruli and tubules of the kidney. We found that the number of vimentin and E-cadherin positive glomeruli and tubules were increased after sunitinib treatment compared to saline treated diabetic mice. The number of vimentin labeled tubules was decreased in the sunitinib treated group compared to diabetic + saline groups. Differences in the number of S100 positive tubules and glomeruli between groups 3 and 4 were not statistically significant. The effect of sunitinib on experimental diabetic mice appears to be related to levels of vimentin, E-cadherin and S100 in the glomeruli and tubules of the kidney, and sunitinib may protect against renal damage from DM.