Prophylactic effect of Biochanin A in lipopolysaccharide-stimulated BV2 microglial cells


Berköz M., Krosniak M., ÖZKAN YILMAZ F., ÖZLÜER HUNT A.

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY, vol.42, no.4, pp.330-339, 2020 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 42 Issue: 4
  • Publication Date: 2020
  • Doi Number: 10.1080/08923973.2020.1769128
  • Journal Name: IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.330-339
  • Keywords: Biochanin A, BV2 microglial cells, neuroinflammation, nuclear factor kappa B, reactive-oxygen species, INFLAMMATORY RESPONSES, ACTIVATION, NEUROINFLAMMATION, INHIBITION, EXPRESSION, NEUROPROTECTION, MACROPHAGES, PHLORETIN, PATHWAY, DISEASE

Abstract

Aim/Purpose of the study:Inhibition of microglial activation using phytochemicals may be a potential candidate for the prevention of neurodegenerative diseases caused by neuroinflammation and oxidative stress. The goal of this study was to investigate the protective role of Biochanin A on lipopolysaccharide (LPS)-stimulated BV2 microglial cells. BV2 microglial cells were treated with LPS in the presence and absence of Biochanin A.Materials and methods:For this aim, nitric oxide production, nuclear factor kappa B (NF-kappa B), tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), IL-6, Prostaglandin E2 (PGE2), and reactive oxygen species (ROS) levels, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), myeloid differentiation factor-88 (MyD88), and toll like receptor-4 (TLR-4) protein expressions, Akt and ERK1/2 phosphorylation levels were measured.Results:Biochanin A pretreatment resulted in significant and concentration-dependently reduced the LPS-induced production of nitric oxide, NF-kappa B p65, TNF-alpha, IL-1 beta, IL-6, PGE2, and ROS compared to the untreated group. Biochanin A prophylaxis exerted an anti-inflammatory effect by suppressing iNOS, COX-2, MyD88, and TLR-4 protein expressions and Akt and ERK1/2 pathway activation.Conclusion:Taken together, these results show that Biochanin A exerts antioxidant and anti-inflammatory activities, thus may be beneficial for preventing neurodegenerative diseases mediated by microglial cells.