Akademik Veri Yönetim Sistemi
3rd International Gazi Pharma Symposium Series (GPSS-2021), Ankara, Türkiye, 8 - 10 Eylül 2021, ss.93
Biofilm-producing bacteria has become a major public health problem worldwide. The
biofilm structure makes the bacteria resistant to the host defense mechanism. Biofilm
plaques detected in many medical devices used in the health sector cause infections arising
from these devices. The biofilm formed by Escherichia coli consists of a bacterial colony
composed of extracellular exopolysaccharide. Colonies from pure bacterial cultures will be
transferred to 100µl TSB and incubated for 18 hours. The 0.5 McFarland standard will be
diluted to obtain turbidity. 100µl will be added to the microplate wells by diluting with TSB
medium. 100µl of 1:100 dilution of the prepared bacterial suspension will be added to these
wells and incubated for 24 hours at 35°C. The wells will be washed 3 times with 200µl
sterile distilled water and dried at 60°C. 200µl of 0.1% crystal violet will be added to dried
microplates and stained for 15 minutes at room temperature. After staining, the wells will
be washed with sterile distilled water and 200µl of 33% glacial acetic acid will be added
after washing and the results will be measured in terms of optical density at 620nm in the
spectrophotometer. Sterile TSB medium will be used for sterility control and wells that do
contain Staphylococcus epidermidis and its formulations will be used for growth control. For
E. coli strains, biofilm formation was interpreted according to the determined control
groups. In conclusion; Low biofilm formation was observed in 10%, moderate in 70% and
high in 20% of the strains.