© 2020, Centenary University. All rights reserved.This study, the effect of ethanol extract of Allium schoenoprasum plant was investigated on some antioxidant parameters and pathogenic microorganisms that affect human health. In this coverage, DPPH (1,1-diphenyl-2-picrilhydrazyl) method was used to determine the antioxidant effect of A. schoenoprasum ethanol extract. Lipid peroxidation prevention activity was evaluated by TBA (Thiobarbituric acid) method. Besides, the antimicrobial activity of plant extract on some pathogenic bacteria was investigated via disk diffusion method. Quercetin was used to determine total flavonoid components. According to our findings, the total flavonoid component amount found in 0.2 mg/ml ethanol extract was found equivalent to quercetin with 44.465 ± 0.204 µg/ml. In addition, A. schoenoprasum showed that it was effective compared to BHA (Beta hydroxy acid), which is the positive control of lipid peroxidation activity. While the lowest and highest concentration values of DPPH radical Scavenging activity were 60,125 - 96,285%, these values were found to be 82.178 - 97.357% for BHA. Ethanol extract of A. schoenoprasum, was determined to show antimicrobial activity against some pathogenic microorganisms such as Bacillus subtilis, Escherichiacoli, Pseudomonas aeruginosa, Enterococcus faecalis, Candida albicans.