International Biochemistry Congress–2017 /28 th National Biochemistry Congress (TurkJBiochem-2017), Erzurum, Turkey, 19 - 23 September 2017, vol.42, no.1, pp.51
OBJECTIVES: This study was planned in order to effect of glutathione known
antioxidant properties the potential oxidative DNA damage and reveals the
effects of antioxidant system in high glucose (HG) added kidney cell line.
MATERIALS-METHODS: BHK-21 cells were cultured with the regular
passages. MTT cell viability was determined for glucose, glutathione. Cells were
seed into plates. Control, glucose (285 mM), GSH (250 µM ) and its cross groups
were prepared . Cells were used for analysis after hours 24 incubation . In these
examples ; 8 -OhdG , TAS , TOS were analyzed by ELISA and
RESULTS: 8-OHdG levels were effected by high glucose (p≤0.05) and, HG plus
and glutathione supplements were increased, too. TOS levels and OSI values in
all working groups (HG, and GSH+HG) increased compared to controls (p≤0.05). The TAS was no effected from glutathione supplemented group . HG
treatment was decreased TAS in TQ and LYC groups (p≤0.05). As a result, in the
HG treated BHK-21 cells, oxidative DNA damage, TOS, OSI increased compared
to the control for all treated groups. Oxidative DNA damage and OSI were
higher than, GSH and, HG plus groups.
CONCLUSIONS : According to the results obtained in the study, no protective
effect of glutathione applied to high glucose cells on the cellular level was
observed at these doses. However , it has also been determined that in
glutathione -administered groups, the doses of glutathione administered are not
toxic doses on the cells.
Keywords: Glucose, cell culture, glutathione, DNA damage, TAS/TOS