The effects of glutathione application on oxidative DNA damage and antioxidant system in glucose-added renal cell line


Yur F. G., Dede S., Taşpınar M., Usta A., Çetin S.

International Biochemistry Congress–2017 /28 th National Biochemistry Congress (TurkJBiochem-2017), Erzurum, Turkey, 19 - 23 September 2017, vol.42, no.1, pp.51

  • Publication Type: Conference Paper / Summary Text
  • Volume: 42
  • City: Erzurum
  • Country: Turkey
  • Page Numbers: pp.51
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

OBJECTIVES: This study was planned in order to effect of glutathione known antioxidant properties the potential oxidative DNA damage and reveals the effects of antioxidant system in high glucose (HG) added kidney cell line. MATERIALS-METHODS: BHK-21 cells were cultured with the regular passages. MTT cell viability was determined for glucose, glutathione. Cells were seed into plates. Control, glucose (285 mM), GSH (250 µM ) and its cross groups were prepared . Cells were used for analysis after hours 24 incubation . In these examples ; 8 -OhdG , TAS , TOS were analyzed by ELISA and spectrophotometric methods. RESULTS: 8-OHdG levels were effected by high glucose (p≤0.05) and, HG plus and glutathione supplements were increased, too. TOS levels and OSI values in all working groups (HG, and GSH+HG) increased compared to controls (p≤0.05). The TAS was no effected from glutathione supplemented group . HG treatment was decreased TAS in TQ and LYC groups (p≤0.05). As a result, in the HG treated BHK-21 cells, oxidative DNA damage, TOS, OSI increased compared to the control for all treated groups. Oxidative DNA damage and OSI were higher than, GSH and, HG plus groups. CONCLUSIONS : According to the results obtained in the study, no protective effect of glutathione applied to high glucose cells on the cellular level was observed at these doses. However , it has also been determined that in glutathione -administered groups, the doses of glutathione administered are not toxic doses on the cells. Keywords: Glucose, cell culture, glutathione, DNA damage, TAS/TOS