Effects of Humic Acid and EDTA on Phytoremediation, Growth and Antioxidant Activity in Rapeseed (Brassica napus L.) Grown under Heavy Metal Stress

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Boysan Canal S., Bozkurt M. A., Yılmaz H.

POLISH JOURNAL OF ENVIRONMENTAL STUDIES, vol.31, no.5, pp.4051-4060, 2022 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 31 Issue: 5
  • Publication Date: 2022
  • Doi Number: 10.15244/pjoes/148120
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Central & Eastern European Academic Source (CEEAS), Environment Index, Greenfile, Public Affairs Index, Veterinary Science Database
  • Page Numbers: pp.4051-4060
  • Van Yüzüncü Yıl University Affiliated: Yes


Rapeseed has been cultivated to produce non-edible and edible oil for thousands of years.

It is known as the second-largest oilseed plant in the world with 24.6 million tons of oil production

in 2021. The interventions that can be carried out during the cultivation of a plant with such

a high production value are quite significant. Growth, enzymatic activities, and phytoremediation of

rapeseed grown under heavy metal stress supported by humic acid (HA) and ethylenediaminetetraacetic

acid (EDTA) applications were investigated for the first time in this study. Three doses of

EDTA (EDTA1:5 mmol/kg, EDTA2:10 mmol/kg, EDTA3:15 mmol/kg) and three doses of HA

(HA1:500 mg/kg, HA2:1000 mg/kg, HA3:2000 mg/kg) were applied in heavy metal treated pots.

According to experiment results, HA1 and HA2 applications increased plant dry and fresh weights,

root dry and fresh weights. However, EDTA applications caused a decrease in shoot length, a number

of leaves, shoot fresh and dry weights, root fresh and dry weights. Bioconcentration factor (BCF)

values for Zn, Cr and Cd we found higher than in both shoots and roots of rapeseed. For all levels of

EDTA, the values of BCF (shoot) and BCF (root), transfer factor (TF) and translocation factor (TLF)

increased compared to HA applications. On the other hand, in comparison to heavy metal polluted

soils alone (PS), all levels of HA resulted in significantly reduced APX and CAT enzyme activity,

and hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents. We concluded that humic

substances exert a significant influence on plant growth and reduce heavy metal toxicity in polluted

soils. At the same time, HA could be more effective than EDTA in terms of phytoremediation of Cr.

HA can increase phytoremediation in polluted soils as it improves plant growth and oxidative stress.