Positive effect of autologous platelet rich plasma on Saanen buck semen cryopreservation in non-breeding season


ALÇAY S., Aktar A., KOCA D., KILIÇ M., Akkasoglu M., SAĞIRKAYA H.

CRYOBIOLOGY, vol.103, pp.45-48, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 103
  • Publication Date: 2021
  • Doi Number: 10.1016/j.cryobiol.2021.09.012
  • Journal Name: CRYOBIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Artic & Antarctic Regions, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.45-48
  • Keywords: Platelet-rich plasma, Buck semen, Cryopreservation, Post-thaw, EGG-YOLK, SOYBEAN LECITHIN, INCUBATION RESILIENCE, OXIDATIVE STRESS, POSTTHAW QUALITY, SPERM, ANTIOXIDANTS, EXTENDERS, FROZEN, FERTILITY
  • Van Yüzüncü Yıl University Affiliated: No

Abstract

The objective of the current study was to evaluate the effect of autologous platelet-rich plasma (PRP) addition into soybean lecithin based extender on buck semen at post-thaw. Semen samples were collected from eight Saanen buck, and each semen sample was split into four equal aliquots and diluted with different concentrations of PRP supplemented extenders [no PRP (control), 0.5 x 107/ml PRP, 1 x 107/ml PRP, or 2 x 107/ml PRP]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed at post-thaw. The results showed that 2 x 107/ml PRP group had a positive effect on motility (62.41 +/- 4.24), membrane functional integrity (71.11 +/- 2.90), mitochondrial membrane potential (69.70 +/- 1.99), DNA integrity (7.22 +/- 0.93) and MDA levels (2.56 +/- 0.73) at post-thaw (P < 0.05). The results of the study demonstrated that autologous PRP has a protective effect on cryopreservation of buck spermatozoa and the fertility effects are worthy of further study.