The objective of this study was to investigate the presence of Listeria species in the corn silage and raw milk samples. A total of 140 raw milk samples obtained from cows, sheeps and goats fed with silage and 90 corn silage samples collected from 10 dairy farms in South-Eastern Region of Turkey were analyzed for Listeria spp. In the result, L. monocytogenes and L. innocua were isolated from 2 (2.2%) and 5 (5.5%) silage samples and from 3 (2.1%) and 5 (3.5%) raw milk samples, respectively. The results indicates that these are a potential risk for animals and public health. Prevention of growth of L. monocytogenes in silage will also contribute to reduction of Listeria spp. in milk.