The inhibitory impact of glutathione (GSH) and ascorbic acid (vitamin C) compounds on glucose-6-phosphate dehydrogenase (G6PD) enzyme purified from sheep liver

Baş Z., Türkoğlu V.

Archives of Physiology and Biochemistry, 2025 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1080/13813455.2025.2567343
  • Dergi Adı: Archives of Physiology and Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Ascorbic acid (vitamin C), glucose-6-phosphate dehydrogenase (G6PD), inhibition, purification, reduced glutathione (GSH)
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Objective: Glucose-6-phosphate dehydrogenase (G6PD) is an enzyme with many essential biochemical functions. However, in various cancer diseases, increased activity of G6PD causes cancer cells to grow, so G6PD inhibitors have become a significant area of research in cancer treatment. Materials and methods: Here, G6PD was purified 4530-fold with affinity chromatography using 2’,5′-ADP Sepharose 4B from sheep liver. The effects of reduced glutathione (GSH) and ascorbic acid (vitamin C) on G6PD activity were explored. Results and discussion: GSH and ascorbic acid showed a significant inhibitory effect on G6PD, and IC50 values were found as 0.37 µM and 34.66 µM, respectively. The inhibition type from Lineweaver-Burk plots of these compounds was identified as non-competitive inhibition. The Ki values of GSH and ascorbic acid were calculated as 0.48 µM and 30.47 µM, respectively. Conclusion: In this study, it was observed that GSH and ascorbic acid antioxidant compounds exhibit an inhibitory effect on G6PD and may be protective and preventive against cancer.