Evaluation of the anticancer potential of a sulphonamide carbonic anhydrase IX inhibitor on cervical cancer cells

Koyuncu İ., Tülüce Y., Qadir H. S., Durgun M., Supuran C. T.

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, vol.34, no.1, pp.703-711, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 1
  • Publication Date: 2019
  • Doi Number: 10.1080/14756366.2019.1579805
  • Journal Name: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.703-711
  • Keywords: Apoptosis, cytotoxicity, cervical cancer, carbonic anhydrase-IX inhibitor, oxidative stress, THERAPEUTIC APPLICATIONS, APOPTOSIS, ANTIOXIDANT, DEATH, E6, DERIVATIVES, EXPRESSION, GENERATION, ROLES, CYCLE
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract


https://www.tandfonline.com/doi/full/10.1080/14756366.2019.1579805

Abstract

Cervical cancer is a common type of cancer. Carbonic anhydrase IX (CA IX) is an attractive target for tumour therapy, being overexpressed in many cancers. We investigated the anticancer properties of the aromatic sulphonamide S-1 as a CA IX inhibitor on cervical cancer cells (HeLa) positive for CA IX expression and normal prostate epithelial cell line (PNT1-A) negative for CA IX. We examined the cytotoxic, apoptosis, genotoxic, and oxidative stress activity of S-1 on HeLa and PNT1-A cell lines. S-1 induced significant reduction of cell viability, caused apoptosis, and up-regulated ROS production. This decrease in cell survival rate can be attributed to the high level of ROS and apoptosis, which has also been shown to arrest the cell cycle. Our findings indicated that S-1 is more effective on HeLa than PNT1-A. S-1 was able to induce apoptosis of cervical cancer cells and is a possible candidate for future anticancer studies.