The present study determined the effects of pre-slaughter ascorbic acid administration on some physiological indicators and meat quality traits of road transport-stressed lambs and kids. Eighteen lambs and 18 kids were divided into the following three groups: no transportation (T0), 3-h transportation (T3), and ascorbid acid administered at 125 mg/kg before 3-h transportation (T3+C). The liveweight loss and rectal temperature were similar, while there were significant differences among groups for some biochemical parameters in both lambs and kids. Glucose concentrations significantly increased after transportation in lambs (P = 0.005) and kids (P < 0.001). Ascorbic acid had significant effects on serum glucose of lambs. T3 lambs had higher glucose concentrations than did T0 lambs (P < 0.001), while T0 and T3+C lambs were found to have similar concentrations. Oxidative status in lambs and kids, as determined by serum malondialdehyde concentration, did not differ among groups. However, some meat quality traits were negatively influenced by transportation. T3 lambs had a significantly lower muscle glycogen concentration than did T0 and T3+C lambs (P = 0.018), while ultimate pH was similar among groups. Muscle glycogen concentration was significantly lower (P = 0.008) and ultimate pH was higher in T3 and T3+C kids (P = 0.028). T3 and T3+C groups had darker meat (L*) than did T0 groups both in lambs and kids, whereas a*, cooking loss and water-holding capacity were similar among groups. The effect of ascorbic acid on meat toughness was significant and shear force value in T0 lambs was lower than that in T3 lambs (P = 0.009), while T0 and T3+C lambs were found to have similar shear force values. Overall, the administration of ascorbic acid to lambs before transportation could partly reduce the adverse effects of road transportation stress, whereas its effects on kids were found to be insignificant.