The effect of lycopene on autophagy in fluoride toxicity in kidney cells

Usta A., Yüksek V., Çetin S., Dede S.

XXVII. Balkan Clinical Laboratory Federation Meeting (27.BCLF-2019) / National Congress of the Turkish Biochemical Society (TBS-2019) Congress, Antalya, Turkey, 26 - 31 October 2019, vol.44, no.3, pp.45

  • Publication Type: Conference Paper / Summary Text
  • Volume: 44
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.45
  • Van Yüzüncü Yıl University Affiliated: Yes


OBJECTIVES:The aim of this study was to investigate the effect of lycopene on autophagic pathway against toxicity induced by sodium fluoride in renal cell line (NRK-52E). MATERIALS and METHODS:Cells were grown in vitro by regular passages. The IC50 value of NaF and the proliferative concentration of lycopene were determined by MTT. In the study, 4 groups were formed as control (K), fluorine (F), lycopene (L) and fluorine lycopene (FL). 24 hours after the application of fluorine and lycopene to the cells at the specified concentrations, RNA isolation and cDNA synthesis were performed and expression of the autophagic genes was determined by RT-PCR. RESULTS:The proliferation enhancing concentration of lycopene (1 µM) and the IC 50 concentration of NaF (3200 µM) at 24 h were found. In the FL and F groups, Sqstm 1 expression increased 19 and 9 times, Atg5, 9 and 5.5 times, Map11c3a 6 and 5 times, respectively. There was no significant change in other genes. CONCLUSIONS:As a result, it was determined that the fluoride given at IC50 concentration affects the autophagic genes studied and the highest increase occurred in Sqstm 1. It can be concluded that lycopene given alone does not alter the genes much, and in the FL group, fluorine may increase autophagy by inhibiting the proliferative effect of lycopene. Keywords: fluorine, cell culture, lycopene, autophagy