Akademik Veri Yönetim Sistemi
XXVII. Balkan Clinical Laboratory Federation Meeting (27.BCLF-2019) / National Congress of the Turkish Biochemical Society (TBS-2019) Congress, Antalya, Türkiye, 26 - 31 Ekim 2019, cilt.44, sa.3, ss.45
OBJECTIVES:The aim of this study was to investigate the effect of lycopene
on autophagic pathway against toxicity induced by sodium fluoride in renal cell
line (NRK-52E).
MATERIALS and METHODS:Cells were grown in vitro by regular passages.
The IC50 value of NaF and the proliferative concentration of lycopene were
determined by MTT. In the study, 4 groups were formed as control (K), fluorine
(F), lycopene (L) and fluorine lycopene (FL). 24 hours after the application of
fluorine and lycopene to the cells at the specified concentrations, RNA isolation
and cDNA synthesis were performed and expression of the autophagic genes was
determined by RT-PCR.
RESULTS:The proliferation enhancing concentration of lycopene (1 µM) and
the IC 50 concentration of NaF (3200 µM) at 24 h were found. In the FL and
F groups, Sqstm 1 expression increased 19 and 9 times, Atg5, 9 and 5.5 times,
Map11c3a 6 and 5 times, respectively. There was no significant change in other
genes.
CONCLUSIONS:As a result, it was determined that the fluoride given at IC50
concentration affects the autophagic genes studied and the highest increase
occurred in Sqstm 1. It can be concluded that lycopene given alone does not
alter the genes much, and in the FL group, fluorine may increase autophagy by
inhibiting the proliferative effect of lycopene.
Keywords: fluorine, cell culture, lycopene, autophagy