Characterization of Staphylococcus species by SDS-PAGE of whole-cell and extracellular proteins


Berber I., Cokmus C., Atalan E.

MICROBIOLOGY, vol.72, no.1, pp.42-47, 2003 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 72 Issue: 1
  • Publication Date: 2003
  • Doi Number: 10.1023/a:1022221905449
  • Title of Journal : MICROBIOLOGY
  • Page Numbers: pp.42-47

Abstract

In this study, a total of fifteen staphylococcal strains belonging to different species were characterized by whole-cell and extracellular protein profiles using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results are presented as dendrograms after quantitative analysis of the band patterns with a computer program. Visual inspection of protein bands and cluster analysis of protein patterns of to be used 15 strains, representing 10 Staphylococcus species, showed that whole-cell and extracellular protein profiles differed in several protein bands in Staphylacoccus aureus, S. epidermidis, S. simulans, and other species of Staphylococcus; however, the differences were insufficient for reliable differentiation of Staphylococcus species by the SDS-PAGE method.