Evaluation of the chemopreventive effects of Ankaferd Bloodstopper in 7,12-dimethylbenz[a]anthracene-induced oral epithelial dysplasia

Ozle M., Cankal D. A. U., Ilhan M., Keles H., Akkol E. K.

CLINICAL ORAL INVESTIGATIONS, vol.22, no.9, pp.3091-3096, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 9
  • Publication Date: 2018
  • Doi Number: 10.1007/s00784-018-2399-9
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.3091-3096
  • Van Yüzüncü Yıl University Affiliated: Yes


ObjectivesAnkaferd BloodStopper (R) (ABS) is an herbal extract which has been used historically as a hemostatic agent in traditional Turkish medicine. ABS comprises of standardized mixture of herbs Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpinia officinarum, and Urtica dioica. In addition to its hemostatic effects, the herb ABS contains some other biological effects including antioxidant and antitumoral properties. The aim of this study is to investigate the chemopreventive effects of ABS in 7,12-dimethylbenz[a]anthracene (DMBA)-induced oral epithelial dysplasia.Materials and methodsA total of 40 Sprague Dawley rats were randomly divided into four groups. Group 1 animals received DMBA alone, and group 2 animals received both DMBA and Ankaferd. Group 3 animals received ABS alone while group 4 animals served as control group and received only liquid paraffine. All animals were sacrificed, and tissue samples were analyzed histologically at the end of the experimental period (14weeks).ResultsHistological studies have shown that the buccal pouches of animals treated with DMBA alone revealed severe dysplasia while only mild or no dysplasia were noticed in DMBA + ABS group. Ankaferd were administered to animals and control group showed no dysplasia or other oral lesions.ConclusionThe results suggest that Ankaferd Bloodstopper (R) has chemopreventive effect against DMBA-induced oral epithelial dysplasia.Clinical relevanceAnkaferd Bloodstopper (R) could be used as a supportive treatment option of cancer in oral and maxillofacial surgery since it possesses chemopreventive effect.