Determination of the active ingredient amounts of expired albendazole drugs

Atasoy N., Aydın F., Akpolat F., Yücel U. M.

Medicine Science, cilt.13, sa.3, ss.550-556, 2024 (Hakemli Dergi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 3
  • Basım Tarihi: 2024
  • Dergi Adı: Medicine Science
  • Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.550-556
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

In this study, it was aimed to determine the active ingredient amounts of 24 expired and 3 unexpired antiparasitic drugs containing the active ingredient albendazole
using UV-Vis and RP-HPLC techniques. Te drugs were prepared using methanol (MeOH) and 1 N HCl (70:30, v/v) mixture. Te quantitative analyses of
albendazole were carried out at 338 nm in the range of 1.562-75 µg mL-1 (R2=0.9997) in UV-Vis. In RP-HPLC, separations were achieved using Nucleosil 100-
5, C18 column (250 x 4.6 mm, 5µm) in isocratic elution. 0.02 M phosphate buffer (pH 3.0) and MeOH mobile phase (30:70, v/v) were used at a flow rate of 1.0
mL min-1. Detections were conducted in the same concentration range (R2=0.9998) using a PDA detector (210 nm). Te results obtained using both techniques
were evaluated by taking into account the studies in the literature. In this study, it was determined that the loss of active ingredient in veterinary drugs containing
different albendazole active ingredient examined decreased over time and that some drugs containing albendazole active ingredient on the market may have less
active ingredient amount than the acceptable limit values.