RESPIRATORY SYNCYTIAL VIRUS INFECTION INDUCES EXPRESSION OF INDUCIBLE NITRIC OXIDE SYNTHASE, CD3, AND CD8 IN NATURALLY OCCURRING PNEUMONIA IN LAMBS


Yaman T. , Aydemir C.

Acta Veterinaria-Beograd, vol.71, no.2, pp.170-188, 2021 (Journal Indexed in SCI Expanded)

  • Publication Type: Article / Article
  • Volume: 71 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.2478/acve-2021-0015
  • Title of Journal : Acta Veterinaria-Beograd
  • Page Numbers: pp.170-188

Abstract

Respiratory syncytial virus (RSV) is an RNA virus that belongs to the Pneumovirus genus

of the Paramyxoviridae family. The aim of this study was to evaluate the expressions of

inducible nitric oxide synthetase (iNOS), CD3 (pan T cells), and CD8 (cytotoxic T cells)

in lamb lungs naturally infected with RSV using immunohistochemistry (IHC). For this

purpose, 100 pneumonic and 10 control lung tissue samples were taken from lambs

slaughtered in the slaughterhouse after macroscopic examination. The streptavidin–

peroxidase method (ABC) was used for IHC staining, and it revealed RSV positivity

in 18 of 100 examined lungs with pneumonia (18%). These positive cases were then

immunostained for iNOS, CD3, and CD8, and compared to controls. In all these cases,

an increase in iNOS expression (100%) was detected, the higher number of CD3+ T

lymphocytes was detected in 14 (78%) cases while CD8+ T lymphocytes were detected

in five (28%) cases, only. Given the increase of iNOS immunoexpression in all RSVpositive

cases and increase in the number of CD3+ T lymphocytes in most cases, it was

concluded that iNOS and CD3+ T lymphocytes play an important role in the immune

response in lamb pneumonia with naturally occurring RSV infection. With this study,

the role of the mentioned markers was evaluated for the first time in lambs naturally

infected with RSV.