Genomic Scan to Detect QTL Using SNP Markers for Simulated Data by Regression Analysis in Half-Sib Design


Koyun H. , KONCAGÜL S., Okut H.

JOURNAL OF ANIMAL AND VETERINARY ADVANCES, cilt.8, ss.2626-2630, 2009 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 8 Konu: 12
  • Basım Tarihi: 2009
  • Dergi Adı: JOURNAL OF ANIMAL AND VETERINARY ADVANCES
  • Sayfa Sayıları: ss.2626-2630

Özet

The aim of the present study was to conduct a genome-wide screening for QTL (Quantitative Trait Loci) detection using the simulated phenotype and genotype data sets obtained from the QTL-MAS workshop 12. A genome scan was carried out in 45 half-sib families to identify QTL influencing a hypothetical trait. Among six chromosomes, each chromosome with 1000 SNP loci, I I informative markers at least 2 cM apart from one another were chosen based on PICs with highest chi(2)-statistic. Half-sib data were pooled and used simultaneously in the analyses conducted for each chromosome separately. Data were analyzed by generating an F-statistic every I cM on a linkage map by regression of phenotypes on the probabilities of inheriting an allele from the sire. Permutation tests at chromosome-wide significance thresholds were carried out over 1000 iterations. Among six chromosomes, significant putative QTL were detected on chromosome 1 (27 cM), 2 (36 cM), 3 (18 cM), 4 (0 cM) and 5 (96 cM) across the families (alpha = 0.01 and alpha = 0.05). There was no QTL detected, exceeding chromosome-wide significance level of p<0.05 and p<0.01 on chromosome 6.