Evaluation of nested PCR for diagnosis of Cyclospora cayetanensis in a sample of immunosuppressed and diarrheic patients in Turkey


Ekici A., Ünlü A. H. , Yılmaz H., Cengiz Z. T. A. S. , Beyhan Y. E.

PARASITOLOGISTS UNITED JOURNAL, vol.14, no.2, pp.141-145, 2021 (ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 14 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.21608/puj.2021.55459.1101
  • Journal Name: PARASITOLOGISTS UNITED JOURNAL
  • Journal Indexes: Emerging Sources Citation Index (ESCI)
  • Page Numbers: pp.141-145
  • Keywords: Cyclospora cayetanensis, diarrhea, immune suppressed, modified acid-fast staining, nested PCR, OUTBREAK
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Background: Cyclospora cayetanensis is a food-borne coccidian parasite that causes cyclosporiasis in humans and possibly in other animals. It presents with watery diarrhea and other related symptoms. Since detection of oocysts may be difficult with histological stains, a negative result should not exclude the possibility of C. cayetanensis. PCR methods can achieve more sensitive detection of the parasite.