Miseq sequence identification of bacteria isolated from Çıldır Lake


Önalan Ş., Seçkin H.

Eastern Anatolian Journal of Science, vol.9, no.2, pp.11-15, 2021 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 9 Issue: 2
  • Publication Date: 2021
  • Journal Name: Eastern Anatolian Journal of Science
  • Journal Indexes: Other Indexes
  • Page Numbers: pp.11-15
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract


 In this study, it was aimed to identify and phylogenetic evaluation of bacteria isolated from Çıldır lake sediment. Four sediment sampling were done from two different regions. For bacterial isolations, samples were incubated in Bennet's agar at 28 oC for 7 days. Morphological and microscopic (Scanning Electron Microscopy-SEM) images of the isolated bacteria were taken. After the PCR process performed with universal primers, the isolated bacteria were identified by sequencing the PCR amplicons using the MiSeq sequence method. In the results of working; 2 Nocardia sp and 1 Amycolatopsis sp species were isolated. When looking at the spore chain morphology in the SEM image, it was observed that a knobby structure was formed, the sequence alignment of 16S rRNA longer than 1000 bp and the sequences obtained from GenBank in the phylogenetic tree were closely similar (99 % <). It has been observed that the bacterial fauna of Çıldır Lake has a low bacterial density that does not pose a risk. The high accuracy of the sequence analysis process for the identification of bacteria has been confirmed. We believe that entering the sequence data of the isolated bacterial species as GenBank data set will be important in terms of providing data for other studies with nucleotide sequences.