The Effect of Diplotaenia turcica Root Extract on Antioxidant Activity and Sperm Parameters in Type 2 Diabetic Rats
Türk Tarım - Gıda Bilim ve Teknoloji dergisi, cilt.14, sa.4, ss.1038-1043, 2026 (TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 14 Sayı: 4
- Basım Tarihi: 2026
- Doi Numarası: 10.24925/turjaf.v14i4.1038-1043.8466
- Dergi Adı: Türk Tarım - Gıda Bilim ve Teknoloji dergisi
- Derginin Tarandığı İndeksler: CAB Abstracts, Central & Eastern European Academic Source (CEEAS), Directory of Open Access Journals, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.1038-1043
- Van Yüzüncü Yıl Üniversitesi Adresli: Evet
Özet
This study aimed to evaluate the effects of Diplotaenia turcica root extract, an endemic plant species of Türkiye, on sperm quality parameters and testicular antioxidant activity in rats with streptozotocin-induced type 2 diabetes. A total of 42 male Wistar rats were randomly and equally allocated into six groups: Control, Diabetes, Diabetes + Diplotaenia turcica ethanol extract, Diabetes + Diplotaenia turcica aqueous extract, Diplotaenia turcica ethanol extract, and Diplotaenia turcica aqueous extract. The control group was fed a standard pellet diet for two weeks and received 10 mL/kg of normal saline orally. In the Diabetes, Diabetes + Diplotaenia turcica ethanol extract, and Diabetes + Diplotaenia turcica aqueous extract groups, type 2 diabetes was induced by feeding a high-fat diet for two weeks, followed by an intraperitoneal injection of streptozotocin dissolved in citrate buffer. The Diplotaenia turcica treatment groups received 150 mg/kg of the respective extract orally for two weeks, according to the experimental design. At the end of the experimental period, blood and sperm samples were collected under anesthesia. In the diabetic group, significant decreases in sperm count and motility were observed, along with a significant increase in abnormal sperm morphology (p<0.001). In addition, malondialdehyde levels in testicular tissue were elevated, whereas glutathione levels and catalase activity were significantly reduced (p<0.05). In the diabetic groups treated with Diplotaenia turcica, improvements in sperm parameters and significant increases in antioxidant enzyme activities were observed (p<0.05). These findings suggest that Diplotaenia turcica root extract may serve as a potential protective agent against diabetes induced sperm abnormalities and testicular oxidative damage.