Lipases perform essential roles in the digestion, transportation and processing of the dietary lipids. The properties of the clay activated carbon (pear shell) and acacia tree (acacia tortilis) as a support for enzyme immobilization were studied using the enzyme lipase. The Km and Vmax values of the butyrate hydrolysis were determined, respectively. The free and immobilized ones on activated carbon and acacia tortilis lipase was determined to be the most active in mildly alkaline conditions (pH 8.0-9.0) and in the temperature range of 40-50 degrees C. Taking in the account the Lagergren Firs-order equation for adsorption kinetics, q1 and k1 which are the measure of adsorbed assets strength have been carried out. Thermodynamic parameters Delta G0, Delta H0 and Delta S0 were calculated.