Immunohistochemical detection of Brucella melitensis antigens in cases of naturally occurring abortions in sheep


Ilhan F. , Yener Z.

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, cilt.20, ss.803-806, 2008 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 20 Konu: 6
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1177/104063870802000616
  • Dergi Adı: JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
  • Sayfa Sayıları: ss.803-806

Özet

Brucella melitensis, a worldwide zoonotic pathogen, is a significant cause of abortion in sheep and goats in some countries. The present Study was carried out to determine, by immunohistochemistry, the presence of B. melitensis antigens in 110 naturally occurring aborted sheep fetuses. Sections Of lung, liver, kidney, and spleen of each fetus were stained with immunoperoxidase to detect Brucella antigens. Brucella melitensis antigens were detected in 33 of 110 fetuses (30%). In the 33 positive cases, Brucella antigens were found in lung (25 [22.7%]), liver (21 [19%]), spleen (13 [11.8%]), and kidney (6 [5.4%]). Microscopic Studies demonstrated that Brucella antigens were mainly located in the cytoplasm of macrophages and neutrophils of the lung, and in the cytoplasm or macrophages in the portal infiltrates and Kupffer cells of the liver. It was Concluded that immunohistochemistry in formalin-fixed, paraffin-ern bedded tissues is a useful tool for the diagnosis of spontaneous ovine abortion caused by B. melitensis.