Preliminary In-vitro Tissue Culture Studies on Devegülü, A Medicinal Plant from Van

I. International Agricultural Science Congress, Van, Türkiye, 9 - 12 Mayıs 2018, ss.31, (Özet Bildiri)

Hollyhock known as Devegülü is a member of Malvaceae, a

perennial herbaceaous plant range-expanding on steppes of 1750-2200m

altitudes in Turkey, Northern Iraq, and North-west Iran. Malvaceae, with

approximately 1500 species used as medicinal plants in Anatolia, is a large

family spreading all over the world except for poles. Local people use the

aerial parts as analgesic, diuretic and for arthritis, kidney and bladder stones.

The purpose of this study was to optimize in vitro conditions for

callogenesis and organogenesis of Devegülü (Hollyhock) using different

explant sources. Various sterilization protocols were applied to Devegülü

plants potting from nature (Konalga, Çatak, Van). The leaves, petioles and

nodes were used as explant sources. Three different concentrations of

Sodium Hypochloride (5 %, 10 % and 15 %) and four different exposure

time (5, 10, 15 and 20 minutes) were tested for surface sterilization.

Explants were cultured on MS basal medium with plant hormones (BAP and

NAA). Callus formation was not observed because of high contamination.

Therefore, Devegülü seeds which were harvested in August (2017) will be

germinated under sterile conditions and then plantlets will be used to obtain

sterile explants for our next studies to establish an efficient regeneration

system.

Keywords: Devegülü, Hollyhock, Medicinal plant, Malvaceae