Introduction Developing new anti-cancer agents are crucial for cancer treatment. Antiproliferative activity of L1H as bis structured schiff base was subjected to preliminary research in eight different kinds of cell lines by cell viability method using different concentrations of it to determine their inhibitory concentration. L1H demonstrated the highest cytotoxicity in human breast cancer cell line MCF-7.

Material and methods In this perspective, the MCF-7 cell line was cultured for the examination of different molecular techniques including MTT, apoptosis analysis by ELISA, comet assay. Moreover, DNA ladder, AO/EB as another apoptotic cell analysis, markers of oxidative stress, and total antioxidant status, total thiol and GSH as non-enzymatic antioxidants assay were conducted.
Results and discussions The above techniques have proven that L1H is a better anticancer drug when compared to cisplatin as a positive control in human breast cancer cells, especially those affected by L1H. The findings clearly show that L1H evaluated in MCF-7 cell lines cause to rising or induced apoptosis, DNA damage, diminished antioxidant status against the increase of oxidised protein, and prevent cell proliferation.
Conclusion Manifold evidences supported our hypothesis that L1H has a potential therapeutically improved against the MCF-7 cell line, and then without doubt to be a suitable candidate drug for investigating cancers next.