Akademik Veri Yönetim Sistemi
South African Journal Of Botany, cilt.142, sa.142, ss.421-429, 2021 (SCI-Expanded)
The use of Salvia species among the public and their importance in the scientific world increase due to their
numerous pharmacological and biological activities on a daily basis. In this study, the phytochemical contents of
different parts (root, branch, leaf, flower, whole) of different specimens of Salvia siirtica (SS) (in vivo and in vitro)
extracts prepared with various solvents (petroleumether, chloroformand ethanol) were determined using different
techniques (GC-MS and LC-MS/MS) and the results were compared. In addition, biological activities (antioxidant,
cytotoxic, anticholinesterase antiurease, antityrosinase, antielastase and anticollagenase) of all samples
were determined and compared. The antioxidant potential of the analysed samples was found to be high, and
their enzyme activity potential was low. Besides, in vitro SS-TIS (Temporary Immersion System) sample showed
high cytotoxic activity (viability% 2.12 § 0.06) against MCF-7 (breast cancer) cell line. The results of GC-MS and
LC-MS/MS analyses indicated that ferruginol and sugiol could be isolated from the ethanol extract of S. siirtica
roots, salvigenin and b-sitosterol from the chloroform extract of the aerial parts, and phenolic compounds from
the ethanol extract of the aerial parts. In addition, the amount of rosmarinic acid, caffeic acid and 12-demethylmulticauline
of in vitro samples were found to be higher than those of in vivo samples. Furthermore, all samples,
both in vivo and in vitro, contained high levels of rosmarinic acid and b-sitosterol. The whole chloroform extract
(SSWC) could be the source extract for salvigenin (33952.13 mg/g) and b-sitosterol (16369.71 mg/g), and the root
ethanol extract (SSRE) for ferruginol (17721.99 mg/g). As a result, it is understood that the nature of the plant
material, the choice of an appropriate solvent and the parts of the material used are quite effective in chemical
content. S. siirtica promise potential natural antioxidant agent in food and/or pharmaceutical industry due to its
phytochemical content (rosmarinic and caffeic acids, ferruginol, salvigenin) and antioxidant activity.