The effectiveness of a CpG motif-based adjuvant (CpG ODN 2006) for LHRH immunization


CONFORTI V. A., DE AVILA D. M., CUMMINGS N. S., WELLS K. J., Uelker H., REEVES J. J.

VACCINE, vol.25, no.35, pp.6537-6543, 2007 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 35
  • Publication Date: 2007
  • Doi Number: 10.1016/j.vaccine.2007.05.056
  • Journal Name: VACCINE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.6537-6543
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

A recombinant ovalbumin-luteinizing hormone-releasing hormone (ova-LHRH) antigen has been developed for immunocontraception. In this study, a novel immunostimulant for ova-LHRH immunization, CpG oligodeoxynucleotide (ODN) 2006, was compared against Mycobacterium butyricum. Also, the immunogenicity of ova-LHRH after lyophilization and exposure to organic solvents was assessed. Rats received either ova-LHRH solubilized in urea; lyophilized ova-LHRH; lyophilized ova-LHRH exposed to methylene chloride; or lyophilized ova-LHRH exposed to ethyl acetate. Immunogenicity of lyophilized ova-LHRH was reduced compared with solubilized ova-LHRH. Exposure to ethyl acetate further decreased immunogenicity of ova-LHRH. CpG ODN 2006 was a more effective immunostimulant than M. butyricum for LHRH immunization. (C) 2007 Elsevier Ltd. All rights reserved.