The effectiveness of a CpG motif-based adjuvant (CpG ODN 2006) for LHRH immunization


CONFORTI V. A. , DE AVILA D. M. , CUMMINGS N. S. , WELLS K. J. , Uelker H. , REEVES J. J.

VACCINE, cilt.25, ss.6537-6543, 2007 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 25 Konu: 35
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.vaccine.2007.05.056
  • Dergi Adı: VACCINE
  • Sayfa Sayıları: ss.6537-6543

Özet

A recombinant ovalbumin-luteinizing hormone-releasing hormone (ova-LHRH) antigen has been developed for immunocontraception. In this study, a novel immunostimulant for ova-LHRH immunization, CpG oligodeoxynucleotide (ODN) 2006, was compared against Mycobacterium butyricum. Also, the immunogenicity of ova-LHRH after lyophilization and exposure to organic solvents was assessed. Rats received either ova-LHRH solubilized in urea; lyophilized ova-LHRH; lyophilized ova-LHRH exposed to methylene chloride; or lyophilized ova-LHRH exposed to ethyl acetate. Immunogenicity of lyophilized ova-LHRH was reduced compared with solubilized ova-LHRH. Exposure to ethyl acetate further decreased immunogenicity of ova-LHRH. CpG ODN 2006 was a more effective immunostimulant than M. butyricum for LHRH immunization. (C) 2007 Elsevier Ltd. All rights reserved.