The effect of quinoa (Chenopodium quinoa willd) on necrotic cell death in rats with insülin resistance


Usta A. , Dede S. , Yüksek V. , Çetin S.

9.Internatıonal Moleculer Bıology and Bıotechnology Congress (Molbiotech), Kars, Türkiye, 6 - 10 Aralık 2020, cilt.1, ss.60

  • Cilt numarası: 1
  • Basıldığı Şehir: Kars
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.60

Özet

Necrosis is induced from cell death types by the activation of Ripk1 (Receptor interacting protein kinases),

which are formed as a result of cellular stress. This study was planned to investigate the effect of quinoa

application on the Ripk1 gene one of the necrotic markers, in rats with experimental insulin resistance by

applying glucocorticoid. For this purpose, 42 male Wistar-Albino rats weighing 200-250 g were used. Six

experimental groups, each consisting of 7 rats; control (C), quinoa given (Q), insulin resistance induceddexamethasone

(D), insulin resistance created and quinoa given (DQ), insulin resistance created and metformin

given (DM), quinoa given and insulin resistance created (QD) groups were formed. D, DQ, DM, QD group rats

were administered dexamethasone (1mg / kg / day) for 7 days to cause insulin resistance. Group D was sacrificed

at the end of 7 days. Then, quinoa feed was given to DQ and metformin was given 40 mg / kg / day to DM, for 6

days. For QD, quinoa feed for 6 days and then insulin resistance (7 days) was created with dexamethasone. The

control group received physiological serum for 7 days, and the quinoa group received quinoa for 6 days. At the

end of the experiment, the animals were sacrificed and their livers were taken. RNA was obtained from liver

tissues and converted into cDNA. Then, Ripk1 gene expression levels of the groups were determined by RTqPCR

method. Accordingly, one of the necrotic genes, Ripk1 was found to be 10-fold upregulated in the insulin

resistance (D) group, 7-fold in the quinoa treatment (DQ) group, 6-fold in the quinoa protection (QD) group, 3-

fold in the treatment control (DM) group. Expression in the quinoa (Q) group was found to be lower than the

control. As a result, it was determined that the necrotic damage in the liver caused by increased glucose in

experimental insulin resistance decreased with the application of quinoa.