Investigation of necrotic genes of sodium fluoride (NaF) in renal cell line


Urut F., Dede S., Yüksek V., Çetin S., Usta A., Taşpınar M.

26th Meeting of Balkan Clinical Laboratory Federation (26.BCLF-2018), Skopje, Macedonia, 3 - 05 October 2018, pp.30

  • Publication Type: Conference Paper / Summary Text
  • City: Skopje
  • Country: Macedonia
  • Page Numbers: pp.30

Abstract

Aim: Kidneys are the organs that play an important role in fluorine metabolism. This study was planned to investigate the possible role of NaF administration in the NRK-52 kidney line, which causes certain levels of cytotoxicity. Methods: Rat renal epitelial NRK-52E (ATCC® CRL-1571™) cell line was cultured in standard cell culture conditions. NaF concentrations were prepared in the medium (50, 100, 250, 500, 1000, 2000, 5000, 7500, 10000 and 20000 μM). MTT assay was performed to detect the cytotoxic effect of NaF in NRK-52E cells. The cells treated with NaF at IC50 value dose were divided into 3 groups on time (3, 12 and 24th hours). Then, total RNA isolation followed by cDNA isolation was performed. Expressions of the necrotic genes (RIP 1 and RIP 3) were determined by RealTime-PCR. Results: Ripk1 gene was decreased at 3 and 12 hours and increased at 24 hours. A significant difference was detected between groups in terms of gene expression pattern. Ripk3 was decreased at 3 hours and increased at 12 and 24 hours. The change in the 24 hours was significantly higher than in the other groups. Conclusions: In conclusion, this study was conducted to elucidate the cause of NaF-induced cell death in renal epithelial cells (NRK-52E). It has been suggested that genes involved in necrotic mechanisms are gradually up-regulated and necrotic genes become more active.