Determination of phylogenetic relations of species belonging to the genus dodder (Cuscuta spp.) that problem in agricultural and non-agricultural lands in Eastern Anatolia (Turkey).


Keskin F., Kaya İ., Usta M., Demir İ., Sıpahıoglu H. M., Nemlı Y.

7th International Weed Science Congress, Praha, Czech Republic, 19 - 25 June 2016, pp.23

  • Publication Type: Conference Paper / Summary Text
  • City: Praha
  • Country: Czech Republic
  • Page Numbers: pp.23
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Dodder species (Cuscuta spp.) are parasitic plants that are very difficult to control and therefore impose a serious threat to agricultural production world-wide. There are 16
species belonging to the genus
Cuscuta in agricultural and non-agricultural lands in Turkey. The seeds of dodder can be spread through contaminated crop seed lots and they remain viable in soil for at least 15 years. The aim of this study was to identify the Cuscuta species that are posing a problem in agricultural and non-agricultural lands in Eastern Anatolia Region of Turkey. Herbarium specimens of each sample have been deposited and identified. In order to determine the phylogenetic relations of species belonging to the genus Cuscuta, DNA isolation has been made from the body tissue of each sample. Conserved regions on ribosomal DNA (rDNA) have been amplified by universal primers via PCR method and have been cloned in a proper cloning vector. The cloned DNA fragments were sequenced and analyzed using the Vector NTI Advance™ and CLC Main Workbench Software to trim vector sequences. The identity of cloned fragments was determined by comparison to published sequence information in NCBI database using the basic local alignment search tool programs. The present study identified 8 taxa belonging to the Cuscuta genus to be present in Eastern Anatolia. The results of the genetic analysis supported the results of morphological detections.