Investigation of 8-OHdG, CYP1A, HSP70 and transcriptional analyses of antioxidant defence system in liver tissues of rainbow trout exposed to eprinomectin

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ALAK G., Yeltekin A. Ç. , Tas I. H. , UÇAR A., PARLAK V., TOPAL A., ...More

FISH & SHELLFISH IMMUNOLOGY, vol.65, pp.136-144, 2017 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 65
  • Publication Date: 2017
  • Doi Number: 10.1016/j.fsi.2017.04.004
  • Title of Journal : FISH & SHELLFISH IMMUNOLOGY
  • Page Numbers: pp.136-144


Eprinomectin (EPM), a member of avermectin family, is a semi-synthetic antibiotic. It has been known that avermectin family enters the aquatic environments and adversely affects the aquatic organisms. Effects of EPM is fully unknown in aquatic organisms especially fish, thus the aim of the present study was to investigate transcriptional changes (sod, cat, gpx) and activities of some antioxidant enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) and malondialdehyde (MDA) levels, oxidative DNA damage (8-hydroxy-2-deoxyguanosine (8-OHdG)) and transcriptional changes of heat shock protein 70 (HSP70), and cytochromes P4501A (CYP1A) in liver tissues of rainbow trout exposed to sublethal EPM concentration (0.001 mu g/L, 0.002 mu g/L, 0.01 mu gL, 0.05 mu g/L) for 24 h, 48 h, 72 h and 96 h.