Investigation of the role of CYP1A1 and CYP1B1 expressions in obesity susceptibility

Polat F., Buluş H., Kaygın P., Dirican O., Şimşek G. G., Yılmaz Sarıaltın S., ...More

Türk Doğa ve Fen Dergisi, vol.11, no.3, pp.69-78, 2022 (Peer-Reviewed Journal)


Obesity, a chronic inflammatory disease, needs to be understood with a more multidisciplinary perspective based on metabolic functioning. The rapid increase in the incidence and prevalence of the disease in recent years has caused it to become a global health problem. CYP1A1 is a key enzyme in the metabolization of several exogenous and endogenous substrates including polyunsaturated fatty acids. CYP1B1 plays a regulatory role in metabolic pathways, such as the metabolism of steroid hormones and fatty acids. Therefore, the purpose of our study is to reveal the role of cytochrome p450 (CYP1A1 and CYP1B1) isozymes in obese patients. In addition, this is the first study to examine the effects of CYP1A1 and CYP1B1 in obese patients concerning various hormones, lipids, and also diabetic and whole blood parameters. Patients with obesity who underwent bariatric surgery were included in our study and the expression of CYP1A1 and CYP1B1 in surgery tissues was determined by immunohistochemistry. CYP1A1 was weakly expressed in 33.3%, moderately in 32.5%, and strongly in 21.4% of tissues. Weak CYP1B1 expression was observed only in 28.6% of the tissues while moderate or strong expression was not observed in any of the tissues. No statistically significant differences were found between expression levels of CYP1A1 and CYP1B1 isozymes in obese patients and demographic characteristics (p>0.05).Correlation analyses were also performed between the expression levels and the clinical data of the patients. There was a significant correlation between CYP1A1 expression and hemoglobin levels (p<0.05). A significant correlation was found between CYP1B1 expression and insulin as well as HDL levels (p<0.05). These detoxification enzymes could be considered one of the key targets in obesity. However, higher expressions should be elucidated with other studies, especially in the molecular pathway. Moreover, new approaches are needed to evaluate susceptibility of the disease and obtain effective treatments.